Y changes in gene expression and colony morphology. Mol Microbiol 2003, 47(3):745?54. V hov?L, Sov ek V, Hlav ek O, Chernyavskiy O, Stp ek L, Kub ov?L, Palkov?Z: Flo11p, drug efflux pumps, and the extracellular matrix cooperate to form biofilm yeast colonies. J Cell Biol 2011, 194(5):679?87. Chandra J, Kuhn DM, Mukherjee PK, Hoyer LL, McCormick T, Ghannoum MA: Biofilm formation by the fungal pathogen Candida albicans: development, architecture, and drug resistance. J Bacteriol 2001, 183(18):5385?394. Al-Fattani MA, Douglas LJ: Biofilm matrix of Candida albicans and Candida tropicalis: chemical composition and role in drug resistance. J Med Microbiol 2006, 55(8):999?008. Martins M, Uppuluri P, Thomas DP, Cleary IA, Henriques M, Lopez-Ribot JL, Oliveira R: Presence of extracellular DNA in the Candida albicans biofilm matrix and its contribution to biofilms. Mycopathologia 2010, 169(5):323?31. Beauvais A, Loussert C, Prevost MC, Verstrepen K, Latge JP: Characterization of a biofilm-like extracellular matrix in FLO1-expressing Saccharomyces cerevisiae cells. FEMS Yeast Res 2009, 9(3):411?19. Sov ek V, V hov?L, Kuthan M, Palkov?Z: General factors important for the formation of structured biofilm-like yeast colonies. Fungal Genet Biol 2010, 47(12):1012?022. C M, Vachov?L, Palkov?Z: Yeast colony survival depends on metabolic adaptation and cell differentiation rather than on stress defense. J Biol Chem 2009, 284(47):32572?2581. Insenser MR, Hernaez ML, Nombela C, Molina M, Molero G, Gil C: Gel and gel-free proteomics to identify Saccharomyces cerevisiae cell surface proteins. J Proteomics 2010, 73(6):1183?195. Lopez-Villar E, Oligomycin Monteoliva L, Larsen MR, Sachon E, Shabaz M, Pardo M, Pla J, Gil C, Roepstorff P, Nombela C: Genetic and proteomic evidences support the localization of yeast enolase in the cell surface. Proteomics 2006, 6(Suppl 1):S107 118. Pardo M, Monteoliva L, Pla J, Sanchez M, Gil C, Nombela C: Two-dimensional analysis of proteins secreted by Saccharomyces cerevisiae regenerating protoplasts: a novel approach to study the cell wall. Yeast 1999, 15(6):459?72. Wessel D, Flugge UI: A method for the quantitative recovery of protein in dilute solution in the presence of detergents and lipids. Anal Biochem 1984, 138(1):141?43. Bradford MM: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976, 72(1):248?54. Nombela C, Gil C, Chaffin WL: Non-conventional protein secretion in yeast. Trends Microbiol 2006, 14(1):15?1. Lattif AA, Chandra J, Chang J, Liu S, Zhou G, Chance MR, Ghannoum MA, Mukherjee PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/6833145 PK: Proteomics and pathway mapping analyses reveal phase-dependent over-expression of proteins associated withFaria-Oliveira et al. BMC Microbiology 2014, 14:244 http://www.biomedcentral.com/1471-2180/14/Page 9 of29.30.31.32.33.34.35.36. 37.38.39.40. 41.42.43.44. 45.46.47. 48.carbohydrate metabolic pathways in Candida albicans biofilms. Open Proteomic J 2008, 1(1):5?6. Ferreira C, Lucas C: The yeast O-acyltransferase Gup1p interferes in lipid metabolism with direct consequences on the sphingolipid-sterol-ordered domains integrity/assembly. Biochim Biophys Acta 2008, 1778(11):2648?653. Ferreira C, Silva S, van Voorst F, Aguiar C, Kielland-Brandt MC, Brandt A, Lucas C: Absence of Gup1p in Saccharomyces cerevisiae results in defective cell wall composition, assembly, stability and morphology. FEMS Yeast Res 2006, 6(7):1027?038. Gomes AM, Kozlowski EO, Pomi.